Operation Manuals

Use the following links for access to the current versions of the Discipline Operating Manuals for the Colorado Bureau of Investigation Forensic Services

DNA 01 Purpose 

DNA 02 Personnel 

DNA 03 Minimum Accomodations and Environmental Conditions 

DNA 04 Documentation and Handling of Evidence 

DNA 05 Equipment 

DNA 06 Reagents, Chemicals, Kits and Standards 

DNA 07 Safety 

DNA 08 Sampling plan and Sample Selection 

DNA 09 Uncertainty of Measurement 

DNA 10-01 Preparation of Samples for DNA Extraction 

DNA 10-03 Extraction Using AutoMate Prepfiler Express 

DNA 10-04 Concentration or Additional Washes of DNA Extracts 

DNA 10-06 Manual Dilution of DNA Extracts 

DNA 10-10 STR Typing via 3500xL Capillary Electrophoresis 

DNA 10-11 GeneMapper ID-X Analysis of Casework Samples 

DNA 10-12 Binary Interpretation of Autosomal Casework Samples 

DNA 10-13 Interpretation of Male-Specific (YSTR) Casework Samples 

DNA 10-14 Autosomal Statistical Analysis 

DNA 10-15 Male-Specific (YSTR) Statistical Analysis 

DNA 10-16 DNA Quantitation using Quantifiler Trio 

DNA 10-17 DNA Amplification Using GlobalFiler 

DNA 10-18 YSTR DNA Amplification Using Yfiler Plus 

DNA 11-01 CODIS Casework--Administration 

DNA 11-02 CODIS Casework - Analysts 

DNA 11-03 CODIS Familial Searching 

DNA 12-01 Proficiency Testing 

DNA 12-02 Training 

DNA 12-03 Report Writing and Reviews 

DNA 12-04 Subcontracting and Outsourcing 

DNA 13-01 STRMix Interpretation 

DNA 13-02 Analysis of Autosomal Casework Samples Using STRMix 

DNA Appendix A- Report Writing 

DNA Appendix B- Glossary 

DNA Appendix C- Abbreviations and Symbols 

DNA Appendix D- Allele Frequencies 

DNA Appendix E Positive Controls and Allelic Ladders 

DNA Appendix F Large Numbers 

FIR 01 Purpose 

FIR 02 Personnel 

FIR 03 Accommodations and Environmental Conditions 

FIR 04 Documentation and Handling of Evidence 

FIR 05 Equipment 

FIR 06 Reagents, Chemicals, Solvents and Standards 

FIR 07 Safety 

FIR 08 Sampling Plan 

FIR 10-01 Firearm Mechanical Function Examination 

FIR 10-02 Fired Cartridge Case-Fired Shotshell Examination 

FIR 10-03 Fired Bullet-Fragment Examination 

FIR 10-04 Shot-Pellet Examination 

FIR 10-05 Live Ammunition Examination 

FIR 10-06 Gunshot Residue Examination in Muzzle-to-Target Distance Determination 

FIR 10-07 Shot Pattern Examinations in Muzzle-to-Target Distance Determinations 

FIR 10-08 Modified Greiss Test for Nitrite Residues 

FIR 10-09 Dithiooxamide Test for Copper or Nickel Residues 

FIR 10-10 Sodium Rhodizonate Test for Lead Residues 

FIR 10-12 Silencer-Suppressor Examination 

FIR 10-13 Serial Number Restoration 

FIR 10-14 General Toolmark Examination 

FIR 10-15 Shooting Reconstruction Techniques and Trajectory Examination 

FIR 10-16 National Integrated Ballistic Information Network (NIBIN) 

FIR 10-17 Physical Match 

FIR 10-18 Reference Collections 

FIR 10-19 Verification 

FIR 10-20 Firearm Testing 

FIR 9 Uncertainty of Measurement 

FIR Appendix A - Report Writing 

FIR Appendix B - Glossary and Definitions 

FIR Appendix C - Abbreviations and Symbols 

FIR Appendix D - Serial Number Restoration Reagents 

FIR Appendix E - Equipment Maintenance Schedule 

FIR Appendix F - LIMS Workflow for Firearm Testing Performed by NIBIN Tech or Other Personnel

TOX 01 Purpose and Introduction 

TOX 02 Personnel 

TOX 03 Accommodations and Environment Factors 

TOX 04 Handling of Toxicology Evidence 

TOX 05 Instrumentation and Equipment Maintenance 

TOX 06 Reference Material, Reagents, Chemicals, Solvents, and Solid Phase Cartridges 

TOX 07 Safety 

TOX 08 Sampling 

TOX 09 Uncertainty of Measurement 

TOX 10-01 Proficiency Testing 

TOX 10-02 Outsourcing 

TOX 10-03 Method Validation 

TOX 10-04 Case Categories and Workflow 

TOX 10-05 Case Documentation, Review, and Discovery 

TOX 10-06 Quality Control and Acceptance Criteria 

TOX 10-07 Blender Cleaning 

TOX 10-08 Glass Liner (Insert) Cleaning 

TOX 10-09 ELISA--Drugs of Abuse Screening 

TOX 10-09 ELISA--Drugs of Abuse Screening 8 Prong Sampler 

TOX 10-10 Ethanol Analysis by Headspace Gas Chromatography 

TOX 10-11 Barbiturates and Phenytoin Quantitation By GCMS 

TOX 10-12 Cocaine and Metabolites Quantitation by GCMS 

TOX 10-13 Phencyclidine (PCP) Quantitation by GCMS 

TOX 10-15 Sympathomimetic Amines Quantitation by GCMS 

TOX 10-16 Cathinones Screen by GCMS (Retired)

TOX 10-17 Prescription Drug Screen and Confirmation by GCMS 

TOX 10-18 Gamma Hydroxybutyrate (GHB) Screen by LCMSMS 

TOX 10-19 Benzodiazepines and Z-Drugs Quantitation by LCMSMS 

TOX 10-20 Cannabinoids Quantitation By LC-MSMS 

TOX 10-21 Carisoprodol, Meprobamate, and Carbamazepine Quantitation by LCMSMS 

TOX 10-22 Blood Alcohol Concentration (BAC) Retrograde Extrapolation 

TOX 10-23 Expert Disclosure Report 

TOX 10-24 Glassware Cleaning 

TOX 10-25 Carboxyhemoglobin by CO-Oximetry 

TOX 10-26 Pain Management Drugs Quantitation by LCMSMS 

TOX 10-27 Atypical Antipsychotic Drugs by LCMSMS 

TOX Appendix A- Scope 

TOX Appendix B-Abbreviations