Operation Manuals

Use the following links for access to the current versions of the Discipline Operating Manuals for the Colorado Bureau of Investigation Forensic Services

The State of Colorado is committed to providing equitable access to our services to all Coloradans. Our ongoing accessibility effort works towards being in line with the Web Content Accessibility Guidelines (WCAG) version 2.1, level AA criteria. If you require an accessible version of any of these documents, please complete the CDPS Issue Reporting, Accommodation and Translation Request Webform or email CDPS_CBI_Website@state.co.us.


DNA Database Operation Manual

DBS 01 Purpose 

DBS 02 Personnel 

DBS 03 Minimum Accommodations and Environmental Conditions

DBS 04 Documentation and Handling of Samples 

DBS 05 Equipment 

DBS 06 Reagents, Chemicals, Solvents and Standards 

DBS 07 Safety 

DBS 08 Sampling Plan and Sample Selection 

DBS 09 Uncertainty of Measurement 

DBS 10-01 Semi-automated Sample Punch 

DBS 10-02 Manual Sample Punch 

DBS 10-04 Manual Wash 

DBS 10-06 Master Mix Addition 

DBS 10-07 Amplification

DBS 10-08 Electrophoresis Plate Prep 

DBS 10-09 Electrophoresis Prep Denature 

DBS 10-10 Post PCR 

DBS 10-11 Data Analysis 

DBS 10-12 CODIS Upload 

DBS 11-01 CODIS Procedures 

DBS 11-02 Familial Searching 

DBS 12-01 Convicted Offender Accessioning 

DBS 12-02 Arrestee Kit Accessioning 

DBS 12-03 Rejection Notification 

DBS 12-04 Research and Destruction of Arrestee Submissions 

DBS 12-05 Arrestee Expungements 

DBS 12-06 Court-ordered Expungements and Administrative Removals 

DBS 12-07 CODIS Match Administrative Tasks 

DBS 12-08 Arrestee Kit Ordering 

DBS 12-09 Criminal History Marking 

DBS 12-10 Proficiency Testing 

DBS 12-11 STAcS Recording 

DBS 12-13 Arrestee Processing 

DBS 13 ACE-V METHODOLOGY Tenprint Analysis and Comparison 

Digital Evidence (DME) Operation Manual

DME 01 Purpose 

DME 02 Personnel 

DME 03 Accommodations and Environmental Conditions 

DME 04 Documentation and Handling of Evidence 

DME 05 Equipment 

DME 06 Reagents 

DME 07 Safety 

DME 08 Sampling 

DME 09 Uncertainty of Measurement 

DME 10-01 Exam Preparation Workstation 

DME 10-02 Computer Access 

DME 10-03 Physical Inspection 

DME 10-04 Write Protecting Media 

DME 10-05 Wiping Media 

DME 10-06 Hard Drive Removal and BIOS Check 

DME 10-07 Imaging Protocol Using Windows 

DME 10-08 Imaging Protocol Using Linux 

DME 10-09 Imaging a Macintosh Computer 

DME 10-10 Cable Acquistion Protocol 

DME 10-11 Handheld-Mobile Devices 

DME 10-12 Examination and Analysis 

DME 10-13 Image Restoration 

DME 10-15 Video Forensic Procedure 

DME 10-16 Equipment Control and Performance Verification Procedure 

DME 10-17 Mobile Data jTag and Chip off 

DME 10-18 Test and Validation Procedure 

DME 10-19 Forensic Audio Analysis 

DME Appendix B- Note Taking Guide for Computer-Cell Phone Processing 

DME Glossary of Terms and Definitions and Approved Abbreviations 

Reporting procedure 

DNA Operation Manual

DNA 01 Purpose 

DNA 02 Personnel 

DNA 03 Minimum Accomodations and Environmental Conditions 

DNA 04 Documentation and Handling of Evidence 

DNA 05 Equipment 

DNA 06 Reagents, Chemicals, Kits and Standards 

DNA 07 Safety 

DNA 08 Sampling plan and Sample Selection 

DNA 09 Uncertainty of Measurement 

DNA 10-01 Preparation of Samples for DNA Extraction 

DNA 10-03 Extraction Using AutoMate Prepfiler Express 

DNA 10-04 Concentration or Additional Washes of DNA Extracts 

DNA 10-06 Manual Dilution of DNA Extracts 

DNA 10-10 STR Typing via 3500xL Capillary Electrophoresis 

DNA 10-11 GeneMapper ID-X Analysis of Casework Samples 

DNA 10-12 Binary Interpretation of Autosomal Casework Samples 

DNA 10-13 Interpretation of Male-Specific (YSTR) Casework Samples 

DNA 10-14 Autosomal Statistical Analysis 

DNA 10-15 Male-Specific (YSTR) Statistical Analysis 

DNA 10-16 DNA Quantitation using Quantifiler Trio 

DNA 10-17 DNA Amplification Using GlobalFiler 

DNA 10-18 YSTR DNA Amplification Using Yfiler Plus 

DNA 11-01 CODIS Casework--Administration 

DNA 11-02 CODIS Casework - Analysts 

DNA 11-03 CODIS Familial Searching 

DNA 12-01 Proficiency Testing 

DNA 12-02 Training 

DNA 12-03 Report Writing and Reviews 

DNA 12-04 Subcontracting and Outsourcing 

DNA 13-01 STRMix Interpretation 

DNA 13-02 Analysis of Autosomal Casework Samples Using STRMix 

DNA Appendix A- Report Writing 

DNA Appendix B- Glossary 

DNA Appendix C- Abbreviations and Symbols 

DNA Appendix D- Allele Frequencies 

DNA Appendix E Positive Controls and Allelic Ladders 

DNA Appendix F Large Numbers 

Drug Chemistry Operation Manual

CHE 01 Purpose 

CHE 02 Personnel 

CHE 03 Accommodations and Environmental Factors 

CHE 04 Handling of Evidence 

CHE 05 Equipment 

CHE 06 Reagents, Chemicals, Solvents, or Standards 

CHE 07 Safety 

CHE 08 Sampling 

CHE 09 Uncertainty of Measurement 

CHE 10-01 Item Numbering 

CHE 10-02 Determining Weights and Volumes 

CHE 10-06 Color Testing 

CHE 10-07 Marijuana and THC Analysis 

CHE 10-08 Serial Dry Extractions 

CHE 10-09 Standard Drug Mixture Procedure 

CHE 10-10 Reference Material Verification and Inventory Requirements 

CHE 10-11 Derivatization Techniques 

CHE 10-13 Identification and Analytical Sufficiency 

CHE 10-14 Method Validation Procedures 

CHE 10-17 Pharmaceutical Product Identification 

CHE 10-18 Miscellaneous Techniques 

CHE 10-19 General Sample Preparation for Instrumental Analysis 

CHE 10-20 The Quantitation of THC in Plant Material by HPLC-UV-DAD 

CHE Appendix A - Laboratory Report Examples 

CHE Appendix B - Glossary 

CHE Appendix C - Abbreviations 

CHE Appendix D - CBI-FS Method Validation Worksheet 

CHE Appendix E - CBI-FS Validation Schedule 

CHE Appendix F - Pharmaceutical Dosage Shapes 

CHE Appendix G - Chemistry Equipment Maintenance Schedule 

CHE Appendix H - Nicolet 380 Valpro Assessment Example

Evidence Operation Manual

EP1 Evidence Entry and Receiving

 EP2 Evidence Security, Storage and Maintenance 

 EP3 Internal Evidence Transfers 

 EP4 Communication Log 

 EP5 Object Repositories 

 EP6 Packets and Sub-items 

 EP7 Evidence Return 

 EP8 Electronic Submission of Digital Evidence

 Evidence Manual Glossary

EP Appendix A - LIMS Corrections

Firearm and Toolmark Operation Manual

FIR 01 Purpose 

FIR 02 Personnel 

FIR 03 Accommodations and Environmental Conditions 

FIR 04 Documentation and Handling of Evidence 

FIR 05 Equipment 

FIR 06 Reagents, Chemicals, Solvents and Standards 

FIR 07 Safety 

FIR 08 Sampling Plan 

FIR 10-01 Firearm Mechanical Function Examination 

FIR 10-02 Fired Cartridge Case-Fired Shotshell Examination 

FIR 10-03 Fired Bullet-Fragment Examination 

FIR 10-04 Shot-Pellet Examination 

FIR 10-05 Live Ammunition Examination 

FIR 10-06 Gunshot Residue Examination in Muzzle-to-Target Distance Determination 

FIR 10-07 Shot Pattern Examinations in Muzzle-to-Target Distance Determinations 

FIR 10-08 Modified Greiss Test for Nitrite Residues 

FIR 10-09 Dithiooxamide Test for Copper or Nickel Residues 

FIR 10-10 Sodium Rhodizonate Test for Lead Residues 

FIR 10-12 Silencer-Suppressor Examination 

FIR 10-13 Serial Number Restoration 

FIR 10-14 General Toolmark Examination 

FIR 10-16 National Integrated Ballistic Information Network (NIBIN) 

FIR 10-17 Physical Match 

FIR 10-18 Reference Collections 

FIR 10-19 Verification 

FIR 10-20 Firearm Testing 

FIR 9 Uncertainty of Measurement 

FIR Appendix A - Report Writing 

FIR Appendix B - Glossary and Definitions 

FIR Appendix C - Abbreviations and Symbols 

FIR Appendix D - Serial Number Restoration Reagents 

FIR Appendix E - Equipment Maintenance Schedule 

FIR Appendix F - LIMS Workflow for Firearm Testing Performed by NIBIN Tech or Other Personnel

Footwear and Tire Track Operation Manual

FTP 01 Purpose 

FTP 02 Personnel 

FTP 03 Accommodations and Environmental Conditions 

FTP 04 Handling of Evidence 

FTP 05 Equipment 

FTP 06 Reagents. Chemicals. Solvents or Standards 

FTP 07 Safety 

FTP 08 Sampling 

FTP 09 Uncertainty of Measurement 

FTP 10-01 ACE-V Methodology 

FTP 10-02 Acid Yellow 7 

FTP 10-03 Amido Black 

FTP 10-04 Ardrox 

FTP 10-05 Basic Yellow 40 

FTP 10-06 Cyanoacrylate Fuming 

FTP 10-07 DFO (1,8- Diazafluoren-9-one) 

FTP 10-08 Digital Imaging and Processing 

FTP 10-09 1, 2 Indanedione 

FTP 10-10 Iodine 

FTP 10-11 Obtaining Test Impressions of Known Footwear 

FTP 10-12 Obtaining Test Impressions of Known Tires 

FTP 10-13 Impression Evidence Processing 

FTP 10-14 LCV (Leucocrystal Violet) 

FTP 10-15 Ninhydrin 

FTP 10-16 Oil Red O 

FTP 10-17 PDMAC 


FTP 10-19 R.A.Y. (Rhodamine 6G-Ardrox-Basic Yellow 40) 

FTP 10-20 Rhodamine 6G 

FTP 10-21 Sticky-Side Powder 

FTP 10-22 Sudan Black 

FTP 10-23 Technical Working Group Committee 

FTP 10-24 Titanium Dioxide 

FTP 10-25 Wetwop 

FTP 10-26 Electrostatic Lifting Device and Vinyl Cling Film 

FTP 10-27 Footwear Outsole-Tire Tread Design Image Search 

FTP 10-28 Potassium Thiocyanate 

FTP 10-29 SoleMate Footwear Outsole Design Image Search 

FTP Appendix A- Abbreviations and Symbols 

FTP Appendix B- Equipment Maintenance Schedule 

Latent Prints Operation Manual

FIN 01 Purpose 

FIN 02 Personnel 

FIN 03 Accomodations and Environmental Conditions 

FIN 04 Handling and Documentation of Evidence 

FIN 05 Equipment 

FIN 06 Reagents.Chemicals.Solvents or Standards 

FIN 07 Safety 

FIN 08 Sampling 

FIN 09 Uncertainty of Measurement 

FIN 10-01 ACE-V Methodology 

FIN 10-02 Acid Yellow 7 

FIN 10-03 AFIS and IAFIS 

FIN 10-04 Amido Black 

FIN 10-05 Ardrox 

FIN 10-06 Basic Yellow 40 

FIN 10-07 Cyanoacrylate Fuming 

FIN 10-08 Identification of Deceased Persons 

FIN 10-09 DFO (1.8- Diazafluoren-9-one) 

FIN 10-10 Digital Imaging and Enhancement 

FIN 10-11 Gentian Violet- Crystal Violet 

FIN 10-12 Heat Application 

FIN 10-13 1,2 Indanedione 

FIN 10-14 Iodine 

FIN 10-15 Obtaining Known Friction Ridge Detail Standards 

FIN 10-16 Latent Print Processing 

FIN 10-17 Leucocrystal Violet (LCV) 

FIN 10-18 Ninhydrin 

FIN 10-19 Oil Red O 

FIN 10-20 PDMAC 

FIN 10-21 Powders 

FIN 10-22 R.A.Y. (Rhodamine 6G-Ardrox-Basic Yellow 40) 

FIN 10-23 Rhodamine 6G 

FIN 10-24 Sticky -Side Powder 

FIN 10-25 Sudan Black 

FIN 10-26 Technical Working Group Committee 

FIN 10-27 Titanium Dioxide 

FIN 10-28 Wetwop 

FIN 10-29 Lumicyano 

FIN Appendix A- Glossary and Definitions 

FIN Appendix B- Abbreviations and Symbols 

FIN Appendix C - Equipment Maintenance Schedule

FIN Appendix D - Report Wording Guidelines 

FIN Appendix E- Documenting a Reverse Hit 

FIN Appendix F - Documenting an AFIS-NGI Significant Consult

FIN Appendix G - Documenting Trace DNA Collections

Serology (Forensic Biology) Operation Manual

SER 01 Purpose 

SER 02 Personnel 

SER 03 Minimum Accomodations and Environmental Conditions 

SER 04 Documentation and Handling of Evidence 

SER 05 Equipment 

SER 06 Reagents, Chemicals, Solvents and Standards 

SER 07 Safety 

SER 08 Sampling 

SER 09 Uncertainty of Measurement 

SER 10-01 Preparation of Bloodstain Card(s) from Liquid Blood 

SER 10-02 Phenolphthalein Testing for Blood 

SER 10-03 Hematrace® Testing for Blood 

SER 10-04 Examination of Evidence Using an Alternate Light Source 

SER 10-05 AP Spot Testing for Seminal Fluid 

SER 10-06 Microscopic Examination for Spermatozoa 

SER 10-07 PSA Semiquant Testing for Seminal Fluid 

SER 10-08 SALIgAE® Testing for Saliva 

SER 10-09 RSID™-Urine Testing for Urine 

SER 10-10 Urobilinogen Testing for Fecal Material 

SER 10-11 Collection of Trace DNA 

SER 10-12 Evaluation of Hairs for DNA Testing Suitability 

SER 10-13 Preparation of Bone and Teeth for DNA Analysis

SER Appendix A- Report Writing 

SER Appendix B- Glossary and Definitions 

SER Appendix C- Abbreviations and Symbols

Toxicology Operation Manual

TOX 01 Purpose and Introduction 

TOX 02 Personnel 

TOX 03 Accommodations and Environment Factors 

TOX 04 Handling of Toxicology Evidence 

TOX 05 Instrumentation and Equipment Maintenance 

TOX 06 Reference Material, Reagents, Chemicals, Solvents, and Solid Phase Cartridges 

TOX 07 Safety 

TOX 08 Sampling 

TOX 09 Uncertainty of Measurement 

TOX 10-01 Proficiency Testing 

TOX 10-02 Outsourcing 

TOX 10-03 Method Validation 

TOX 10-04 Case Categories and Workflow 

TOX 10-05 Case Documentation, Review, and Discovery 

TOX 10-06 Quality Control and Acceptance Criteria 

TOX 10-07 Blender Cleaning 

TOX 10-08 Glass Liner (Insert) Cleaning 

TOX 10-09 ELISA--Drugs of Abuse Screening 

TOX 10-09 ELISA--Drugs of Abuse Screening 8 Prong Sampler 

TOX 10-10 Ethanol Analysis by Headspace Gas Chromatography 

TOX 10-11 Barbiturates and Phenytoin Quantitation By GCMS 

TOX 10-12 Cocaine and Metabolites Quantitation by GCMS 

TOX 10-13 Phencyclidine (PCP) Quantitation by GCMS 

TOX 10-15 Sympathomimetic Amines Quantitation by GCMS 

TOX 10-16 Cathinones Screen by GCMS (Retired)

TOX 10-17 Prescription Drug Screen and Confirmation by GCMS 

TOX 10-18 Gamma Hydroxybutyrate (GHB) Screen by LCMSMS 

TOX 10-19 Benzodiazepines and Z-Drugs Quantitation by LCMSMS 

TOX 10-20 Cannabinoids Quantitation By LC-MSMS 

TOX 10-21 Carisoprodol, Meprobamate, and Carbamazepine Quantitation by LCMSMS 

TOX 10-22 Blood Alcohol Concentration (BAC) Retrograde Extrapolation 

TOX 10-23 Expert Disclosure Report 

TOX 10-24 Glassware Cleaning 

TOX 10-25 Carboxyhemoglobin by CO-Oximetry (Retired)

TOX 10-26 Pain Management Drugs Quantitation by LCMSMS 

TOX 10-27 Atypical Antipsychotic Drugs by LCMSMS 

TOX 10-28 Reagent Preparation

TOX Appendix A- Scope 

TOX Appendix B-Abbreviations

Trace Operation Manual

TRA 01 Purpose 

 TRA 02 Personnel 

 TRA 03 Environmental Conditions 

 TRA 04 Documentation and Handling of Evidence 

 TRA 05 Equipment 

 TRA 06 Reagents, Chemicals, Solvents and Standards 

 TRA 07 Safety 

 TRA 08 Sampling Plan and Sample Selection 

 TRA 09 Uncertainty of Measurement 

 TRA 10-01 Tape Analysis 

 TRA 10-02 Gunshot Residue Analysis 

 TRA 10-03 Fire Debris and Ignitable Liquids Analysis 

 TRA 10-04 Paint Analysis 

 TRA 10-05 Fiber and General Trace Evidence Collection 

 TRA 10-06 Microspectrophotometer Analysis 

 TRA 10-07 Fiber Analysis-Microscopic and Instrumental Analysis 

 TRA 10-08 Fabrics, Yarns, and Buttons Examinations 

 TRA 10-09 Fabric Damage Analysis 

 TRA 10-10 Cordage Examinations 

 TRA 10-11 General Microscope Operation and Application 

 TRA 10-12 Gas Chromatography-Mass Spectrometry Analysis - General Unknowns 

 TRA 10-13 Scanning Electron Micrsoscopy-Energy Dispersive Spectroscopy Analysis 

 TRA 10-14 Physical Fit 

 TRA 10-15 General Unknown Materials 

 TRA 10-16 Pyrolysis-Gas Chromatography-Mass Spectrometry Analysis of Paints, Tapes, and Polymers 

 TRA 10-17 Fourier Transform Infrared (FTIR) Analysis of Paints, Tape, and Polymers 

 TRA 10-18 X-Ray Diffraction 

 TRA 10-19 Wood 

 TRA 10-20 NIGHTSEA Stereo Microscope Fluorescence Adaptor 

 TRA Appendix A - Conclusion Scales 

 TRA Appendix B - Terminology

Quality Manual

Quality Manual 

 Quality Manual Glossary 

 Externally Controlled Document List 

 QP01 Commitment to Quality 

 QP02 Responsibilities, Authority and Management Continuity 

 QP03 Electronic Document Management System 

 QP04 Management System Review 

 QP05 Document Control 

 QP06 Review of Requests, Tenders and Contracts 

 QP07 Subcontracting, Outsourcing 

 QP08 Reagents, Chemicals and Supplies 

 QP09 Evaluation of Suppliers 

 QP10 Responsibilities to the Customer 

 QP11 Quality Incident Review 

 QP12 Corrective Action 

 QP13 Preventive Action 

 QP14 Control of Records 

 QP15 Audits 

 QP16 Training 

 QP17 Security 

 QP18 Deviations 

 QP19 Validations 

 QP20 Estimating Uncertainty of Measurement 

 QP21 Equipment, Instrumentation 

 QP22 Measurement Traceability 

 QP23 Reference Standards 

 QP24 Reference Materials 

 QP25 Proficiency Testing 

 QP26 Reviews 

 QP27 Testimony 

 QP28 Reporting of Results 

 QP29 Temperature Monitoring 

 QP30 Consumptive Testing and Observation