Operation Manuals & Additional Resources
The State of Colorado is committed to providing equitable access to our services to all Coloradans. Our ongoing accessibility effort works towards being in line with the Web Content Accessibility Guidelines (WCAG) version 2.1, level AA criteria. If you require an accessible version of any of these documents, please complete the CDPS Issue Reporting, Accommodation and Translation Request Webform or email CDPS_CBI_Website@state.co.us.
Use the following links for access to the current versions of the Discipline Operating Manuals for the Colorado Bureau of Investigation Forensic Services:
- DNA Database Operation Manual
- DBS 01 Purpose
- DBS 02 Personnel
- DBS 03 Minimum Accommodations and Environmental Conditions
- DBS 04 Documentation and Handling of Samples
- DBS 05 Equipment
- DBS 06 Reagents, Chemicals, Solvents and Standards
- DBS 07 Safety
- DBS 08 Sampling Plan and Sample Selection
- DBS 09 Uncertainty of Measurement
- DBS 10-01 Semi-automated Sample Punch
- DBS 10-02 Manual Sample Punch
- DBS 10-03 Automated FTA Extraction (Retired)
- DBS 10-04 Manual Wash
- DBS 10-05 Chelex Cutting and Extraction (Retired)
- DBS 10-06 Master Mix Addition
- DBS 10-07 Amplification
- DBS 10-08 Electrophoresis Plate Prep
- DBS 10-09 Electrophoresis Prep Denature
- DBS 10-10 Post PCR
- DBS 10-11 Data Analysis
- DBS 10-12 CODIS Upload
- DBS 11-01 CODIS Procedures
- DBS 11-02 Familial Searching
- DBS 12-01 Convicted Offender Accessioning
- DBS 12-02 Arrestee Kit Accessioning
- DBS 12-03 Rejection Notification
- DBS 12-04 Research and Destruction of Arrestee Submissions
- DBS 12-05 Arrestee Expungements
- DBS 12-06 Court-ordered Expungements and Administrative Removals
- DBS 12-07 CODIS Match Administrative Tasks
- DBS 12-08 Arrestee Kit Ordering
- DBS 12-09 Criminal History Marking
- DBS 12-10 Proficiency Testing
- DBS 12-11 STAcS Recording
- DBS 12-12 CODIS Hit Tracking Administrative Tasks (Retired)
- DBS 12-13 Arrestee Processing
- DBS 13 ACE-V METHODOLOGY Tenprint Analysis and Comparison
- Digital Evidence (DME) Operation Manual
- DME 01 Purpose
- DME 02 Personnel
- DME 03 Accommodations and Environmental Conditions
- DME 04 Documentation and Handling of Evidence
- DME 05 Equipment
- DME 06 Reagents
- DME 07 Safety
- DME 08 Sampling
- DME 09 Uncertainty of Measurement
- DME 10-01 Exam Preparation Workstation
- DME 10-02 Computer Access
- DME 10-03 Physical Inspection
- DME 10-04 Write Protecting Media
- DME 10-05 Wiping Media
- DME 10-06 Hard Drive Removal and BIOS Check
- DME 10-07 Imaging Protocol Using Windows
- DME 10-08 Imaging Protocol Using Linux
- DME 10-09 Imaging a Macintosh Computer
- DME 10-10 Cable Acquisition Protocol
- DME 10-11 Handheld-Mobile Devices
- DME 10-12 Examination and Analysis
- DME 10-13 Image Restoration
- DME 10-14 Report Requirements (Retired)
- DME 10-15 Video Forensic Procedure
- DME 10-16 Equipment Control and Performance Verification Procedure
- DME 10-17 Mobile Data jTag and Chip off
- DME 10-18 Test and Validation Procedure
- DME 10-19 Forensic Audio Analysis
- DME - Note Taking Guide for Computer-Cell Phone Processing
- DME - Glossary of Terms and Definitions and Approved Abbreviations
- DME - Reporting Procedure
- DNA Operation Manual
- DNA 01 Purpose
- DNA 02 Personnel
- DNA 03 Minimum Accomodations and Environmental Conditions
- DNA 04 Documentation and Handling of Evidence
- DNA 05 Equipment
- DNA 06 Reagents, Chemicals, Kits and Standards
- DNA 07 Safety
- DNA 08 Sampling plan and Sample Selection
- DNA 09 Uncertainty of Measurement
- DNA 10-01 Preparation of Samples for DNA Extraction
- DNA 10-02 Organic Extraction using Phenol-Chloroform-Isoamyl Alcohol (Retired)
- DNA 10-03 Extraction Using AutoMate Prepfiler Express
- DNA 10-04 Concentration or Additional Washes of DNA Extracts
- DNA 10-05 DNA Quantification using Quantifiler Duo (Retired)
- DNA 10-06 Manual Dilution of DNA Extracts
- DNA 10-07 DNA Amplification Using Identifiler Plus (Retired)
- DNA 10-08 DNA Amplification Using MiniFiler (Retired)
- DNA 10-09 DNA Amplification Using YFiler (Retired)
- DNA 10-10 STR Typing via 3500xL Capillary Electrophoresis
- DNA 10-11 GeneMapper ID-X Analysis of Casework Samples
- DNA 10-12 Binary Interpretation of Autosomal Casework Samples
- DNA 10-13 Interpretation of Male-Specific (YSTR) Casework Samples
- DNA 10-14 Autosomal Statistical Analysis
- DNA 10-15 Male-Specific (YSTR) Statistical Analysis
- DNA 10-16 DNA Quantitation using Quantifiler Trio
- DNA 10-17 DNA Amplification Using GlobalFiler
- DNA 10-18 YSTR DNA Amplification Using Yfiler Plus
- DNA 11-01 CODIS Casework--Administration
- DNA 11-02 CODIS Casework - Analysts
- DNA 11-03 CODIS Familial Searching
- DNA 12-01 Proficiency Testing
- DNA 12-02 Training
- DNA 12-03 Report Writing and Reviews
- DNA 12-04 Subcontracting and Outsourcing
- DNA 13-01 STRMix Interpretation
- DNA 13-02 Analysis of Autosomal Casework Samples Using STRMix
- DNA Appendix A - Report Writing
- DNA Appendix B - Glossary
- DNA Appendix C - Abbreviations and Symbols
- DNA Appendix D - Allele Frequencies
- DNA Appendix E - Positive Controls and Allelic Ladders
- DNA Appendix F - Large Numbers
- Drug Chemistry Operation Manual
- CHE 01 Purpose
- CHE 02 Personnel
- CHE 03 Accommodations and Environmental Factors
- CHE 04 Handling of Evidence
- CHE 05 Equipment
- CHE 06 Reagents, Chemicals, Solvents, or Standards
- CHE 07 Safety
- CHE 08 Sampling
- CHE 09 Uncertainty of Measurement
- CHE 10-01 Item Numbering
- CHE 10-02 Determining Weights and Volumes
- CHE 10-03 Sampling (Retired)
- CHE 10-04 Analysis of Psilocybin, Psilocin, and Bufotenine (Retired)
- CHE 10-05 LSD and Related Chemicals - Extraction and Analysis (Retired)
- CHE 10-06 Color Testing
- CHE 10-07 Cannabinoid Analysis
- CHE 10-08 Serial Dry Extractions
- CHE 10-09 Standard Drug Mixture Procedure
- CHE 10-10 Reference Material Verification and Inventory Requirements
- CHE 10-11 Derivatization Techniques
- CHE 10-12 Clandestine Drug Laboratory Evidence Analysis (Retired)
- CHE 10-13 Identification and Analytical Sufficiency
- CHE 10-14 Method Validation Procedures
- CHE 10-15 Thin Layer Chromatography (Retired)
- CHE 10-16 Methamphetamine Quantitative Analysis (Retired)
- CHE 10-17 Pharmaceutical Product Identification
- CHE 10-18 Miscellaneous Techniques
- CHE 10-19 General Sample Preparation for Instrumental Analysis
- CHE 10-20 The Quantitation of THC in Plant Material by HPLC-UV-DAD
- CHE Appendix A - Laboratory Report Examples
- CHE Appendix B - Glossary
- CHE Appendix C - Abbreviations
- CHE Appendix D - CBI-FS Method Validation Worksheet
- CHE Appendix E - CBI-FS Validation Schedule
- CHE Appendix F - Pharmaceutical Dosage Shapes
- CHE Appendix G - Chemistry Equipment Maintenance Schedule
- CHE Appendix H - Nicolet iS50 Valpro Assessment Example
- Evidence Operation Manual
- EP1 Evidence Entry and Receiving
- EP2 Evidence Security, Storage and Maintenance
- EP3 Internal Evidence Transfers
- EP4 Communication Log
- EP5 Object Repositories
- EP6 Packets and Sub-items
- EP7 Evidence Return
- EP8 Electronic Submission of Digital Evidence
- EP Appendix A - LIMS Corrections
- EP Appendix B - Evidence Manual Glossary
- Firearm and Toolmark Operation Manual
- FIR 01 Purpose
- FIR 02 Personnel
- FIR 03 Accommodations and Environmental Conditions
- FIR 04 Documentation and Handling of Evidence
- FIR 05 Equipment
- FIR 06 Reagents, Chemicals, Solvents and Standards
- FIR 07 Safety
- FIR 08 Sampling Plan
- FIR 09 Uncertainty of Measurement
- FIR 10-01 Firearm Mechanical Function Examination
- FIR 10-02 Fired Cartridge Case-Fired Shotshell Examination
- FIR 10-03 Fired Bullet-Fragment Examination
- FIR 10-04 Shot-Pellet Examination
- FIR 10-05 Live Ammunition Examination
- FIR 10-06 Gunshot Residue Examination in Muzzle-to-Target Distance Determination
- FIR 10-07 Shot Pattern Examinations in Muzzle-to-Target Distance Determinations
- FIR 10-08 Modified Greiss Test for Nitrite Residues
- FIR 10-09 Dithiooxamide Test for Copper or Nickel Residues
- FIR 10-10 Sodium Rhodizonate Test for Lead Residues
- FIR 10-11 Stippling-Tattooing Examination (Retired)
- FIR 10-12 Suppressor Examination
- FIR 10-13 Serial Number Restoration
- FIR 10-14 General Toolmark Examination
- FIR 10-15 Shooting Reconstruction Techniques and Trajectory Examination (Retired)
- FIR 10-16 National Integrated Ballistic Information Network (NIBIN)
- FIR 10-17 Physical Match
- FIR 10-18 Reference Collections
- FIR 10-19 Verification
- FIR 10-20 Firearm Testing
- FIR 10-21 NIBIN with the use of the NNCTC
- FIR Appendix A - Report Writing
- FIR Appendix B - Glossary and Definitions
- FIR Appendix C - Abbreviations and Symbols
- FIR Appendix D - Serial Number Restoration Reagents
- FIR Appendix E - Equipment Maintenance Schedule
- FIR Appendix F - LIMS Workflow for Firearm Testing Performed by NIBIN Tech or Other Personnel
- Footwear and Tire Track Operation Manual
- FTP 01 Purpose
- FTP 02 Personnel
- FTP 03 Accommodations and Environmental Conditions
- FTP 04 Handling of Evidence
- FTP 05 Equipment
- FTP 06 Reagents. Chemicals. Solvents or Standards
- FTP 07 Safety
- FTP 08 Sampling
- FTP 09 Uncertainty of Measurement
- FTP 10-01 ACE-V Methodology
- FTP 10-02 Acid Yellow 7
- FTP 10-03 Amido Black
- FTP 10-04 Ardrox
- FTP 10-05 Basic Yellow 40
- FTP 10-06 Cyanoacrylate Fuming
- FTP 10-07 DFO (1,8- Diazafluoren-9-one)
- FTP 10-08 Digital Imaging and Processing
- FTP 10-09 1, 2 Indanedione
- FTP 10-10 Iodine
- FTP 10-11 Obtaining Test Impressions of Known Footwear
- FTP 10-12 Obtaining Test Impressions of Known Tires
- FTP 10-13 Impression Evidence Processing
- FTP 10-14 LCV (Leucocrystal Violet)
- FTP 10-15 Ninhydrin
- FTP 10-16 Oil Red O
- FTP 10-17 PDMAC
- FTP 10-18 POWDERS
- FTP 10-19 R.A.Y. (Rhodamine 6G-Ardrox-Basic Yellow 40)
- FTP 10-20 Rhodamine 6G
- FTP 10-21 Sticky-Side Powder
- FTP 10-22 Sudan Black
- FTP 10-23 Technical Working Group Committee
- FTP 10-24 Titanium Dioxide
- FTP 10-25 Wetwop
- FTP 10-26 Electrostatic Lifting Device and Vinyl Cling Film
- FTP 10-27 Footwear Outsole-Tire Tread Design Image Search
- FTP 10-28 Potassium Thiocyanate
- FTP 10-29 SoleMate Footwear Outsole Design Image Search
- FTP Appendix A- Abbreviations and Symbols
- FTP Appendix B- Equipment Maintenance Schedule
- Latent Prints Operation Manual
- FIN 01 Purpose
- FIN 02 Personnel
- FIN 03 Accomodations and Environmental Conditions
- FIN 04 Handling and Documentation of Evidence
- FIN 05 Equipment
- FIN 06 Reagents.Chemicals.Solvents or Standards
- FIN 07 Safety
- FIN 08 Sampling
- FIN 09 Uncertainty of Measurement
- FIN 10-01 ACE-V Methodology
- FIN 10-02 Acid Yellow 7
- FIN 10-03 Database Searches (AFIS/NGI/Case Specific)
- FIN 10-04 Amido Black
- FIN 10-05 Ardrox
- FIN 10-06 Basic Yellow 40
- FIN 10-07 Cyanoacrylate Fuming
- FIN 10-08 Identification of Deceased Persons
- FIN 10-09 DFO (1.8- Diazafluoren-9-one)
- FIN 10-10 Digital Imaging and Enhancement
- FIN 10-11 Gentian Violet- Crystal Violet
- FIN 10-12 Heat Application
- FIN 10-13 1,2 Indanedione
- FIN 10-14 Iodine (Retired)
- FIN 10-15 Obtaining Known Friction Ridge Detail Standards
- FIN 10-16 Latent Print Processing
- FIN 10-17 Leucocrystal Violet (LCV)
- FIN 10-18 Ninhydrin
- FIN 10-19 Oil Red O
- FIN 10-20 PDMAC
- FIN 10-21 Powders
- FIN 10-22 R.A.Y. (Rhodamine 6G-Ardrox-Basic Yellow 40)
- FIN 10-23 Rhodamine 6G
- FIN 10-24 Sticky -Side Powder
- FIN 10-25 Sudan Black
- FIN 10-26 Latent Print Consensus Panel
- FIN 10-27 Titanium Dioxide
- FIN 10-28 Wetwop
- FIN 10-29 Lumicyano
- FIN Appendix A- Glossary and Definitions
- FIN Appendix B- Abbreviations and Symbols
- FIN Appendix C - Equipment Maintenance Schedule
- FIN Appendix D - Report Wording Guidelines
- FIN Appendix E- Documenting a Reverse Hit
- FIN Appendix F - Documenting an AFIS-NGI Significant Consult
- FIN Appendix G - Documenting Trace DNA Collections
- Serology (Forensic Biology) Operation Manual
- SER 01 Purpose
- SER 02 Personnel
- SER 03 Minimum Accomodations and Environmental Conditions
- SER 04 Documentation and Handling of Evidence
- SER 05 Equipment
- SER 06 Reagents, Chemicals, Solvents and Standards
- SER 07 Safety
- SER 08 Sampling
- SER 09 Uncertainty of Measurement
- SER 10-01 Preparation of Bloodstain Card(s) from Liquid Blood
- SER 10-02 Phenolphthalein Testing for Blood
- SER 10-03 Hematrace® Testing for Blood
- SER 10-04 Examination of Evidence Using an Alternate Light Source
- SER 10-05 AP Spot Testing for Seminal Fluid
- SER 10-06 Microscopic Examination for Spermatozoa
- SER 10-07 PSA Semiquant Testing for Seminal Fluid
- SER 10-08 SALIgAE® Testing for Saliva
- SER 10-09 RSID™-Urine Testing for Urine
- SER 10-10 Urobilinogen Testing for Fecal Material
- SER 10-11 Collection of Trace DNA
- SER 10-12 Evaluation of Hairs for DNA Testing Suitability
- SER 10-13 Preparation of Bone and Teeth for DNA Analysis
- SER Appendix A- Report Writing
- SER Appendix B- Glossary and Definitions
- SER Appendix C- Abbreviations and Symbols
- Toxicology Operation Manual
- TOX 01 Purpose and Introduction
- TOX 02 Personnel
- TOX 03 Accommodations and Environment Factors
- TOX 04 Handling of Toxicology Evidence
- TOX 05 Instrumentation and Equipment Maintenance
- TOX 06 Reference Material, Reagents, Chemicals, Solvents, and Solid Phase Cartridges
- TOX 07 Safety
- TOX 08 Sampling
- TOX 09 Uncertainty of Measurement
- TOX 10-01 Proficiency Testing
- TOX 10-02 Outsourcing
- TOX 10-03 Method Validation
- TOX 10-04 Case Categories and Workflow
- TOX 10-05 Case Documentation, Review, and Discovery
- TOX 10-06 Quality Control and Acceptance Criteria
- TOX 10-07 Blender Cleaning
- TOX 10-08 Glass Liner (Insert) Cleaning (Retired)
- TOX 10-09 ELISA--Drugs of Abuse Screening
- TOX 10-09 ELISA--Drugs of Abuse Screening 8 Prong Sampler
- TOX 10-10 Ethanol Analysis by Headspace Gas Chromatography
- TOX 10-11 Barbiturates and Phenytoin Quantitation By GCMS
- TOX 10-12 Cocaine and Metabolites Quantitation by GCMS
- TOX 10-13 Phencyclidine (PCP) Quantitation by GCMS
- TOX 10-14 Opiates Quantitation by GCMS (Retired)
- TOX 10-15 Sympathomimetic Amines Quantitation by GCMS
- TOX 10-16 Cathinones Screen by GCMS (Retired)
- TOX 10-17 Prescription Drug Screen and Confirmation by GCMS
- TOX 10-18 Gamma Hydroxybutyrate (GHB) Screen by LCMSMS
- TOX 10-19 Benzodiazepines and Z-Drugs Quantitation by LCMSMS
- TOX 10-20 Cannabinoids Quantitation By LC-MSMS
- TOX 10-21 Carisoprodol, Meprobamate, and Carbamazepine Quantitation by LCMSMS
- TOX 10-22 Blood Alcohol Concentration (BAC) Retrograde Extrapolation
- TOX 10-23 Expert Disclosure Report
- TOX 10-24 Glassware Cleaning
- TOX 10-25 Carboxyhemoglobin by CO-Oximetry (Retired)
- TOX 10-26 Pain Management Drugs Quantitation by LCMSMS
- TOX 10-27 Atypical Antipsychotic Drugs by LCMSMS
- TOX 10-28 Reagent Preparation
- TOX Appendix A- Scope
- TOX Appendix B-Abbreviations
- Trace Operation Manual
- TRA 01 Purpose
- TRA 02 Personnel
- TRA 03 Environmental Conditions
- TRA 04 Documentation and Handling of Evidence
- TRA 05 Equipment
- TRA 06 Reagents, Chemicals, Solvents and Standards
- TRA 07 Safety
- TRA 08 Sampling Plan and Sample Selection
- TRA 09 Uncertainty of Measurement
- TRA 10-01 Tape Analysis
- TRA 10-02 Gunshot Residue Analysis
- TRA 10-03 Fire Debris and Ignitable Liquids Analysis
- TRA 10-04 Paint Analysis
- TRA 10-05 Fiber and General Trace Evidence Collection
- TRA 10-06 Microspectrophotometer Analysis
- TRA 10-07 Fiber Analysis-Microscopic and Instrumental Analysis
- TRA 10-08 Fabrics, Yarns, and Buttons Examinations
- TRA 10-09 Fabric Damage Analysis
- TRA 10-10 Cordage Examinations
- TRA 10-11 General Microscope Operation and Application
- TRA 10-12 Gas Chromatography-Mass Spectrometry Analysis - General Unknowns
- TRA 10-13 Scanning Electron Micrsoscopy-Energy Dispersive Spectroscopy Analysis
- TRA 10-14 Physical Fit
- TRA 10-15 General Unknown Materials
- TRA 10-16 Pyrolysis-Gas Chromatography-Mass Spectrometry Analysis of Paints, Tapes, and Polymers
- TRA 10-17 Fourier Transform Infrared (FTIR) Analysis of Paints, Tape, and Polymers
- TRA 10-18 X-Ray Diffraction
- TRA 10-19 Wood
- TRA 10-20 NIGHTSEA Stereo Microscope Fluorescence Adaptor
- TRA Appendix A - Conclusion Scales
- TRA Appendix B - Terminology
- Quality Manual
- Quality Manual
- Quality Manual Glossary
- Externally Controlled Document List
- QP 01 Commitment to Quality
- QP 02 Responsibilities, Authority and Management Continuity
- QP 03 Electronic Document Management System
- QP 04 Management System Review
- QP 05 Document Control
- QP 06 Review of Requests, Tenders and Contracts
- QP 07 Subcontracting, Outsourcing
- QP 08 Reagents, Chemicals and Supplies
- QP 09 Evaluation of Suppliers
- QP 10 Responsibilities to the Customer
- QP 11 Quality Incident Review
- QP 12 Corrective Action
- QP 13 Preventive Action
- QP 14 Control of Records
- QP 15 Audits
- QP 16 Training
- QP 17 Security
- QP 18 Deviations
- QP 19 Validations
- QP 20 Estimating Uncertainty of Measurement
- QP 21 Equipment, Instrumentation
- QP 22 Measurement Traceability
- QP 23 Reference Standards
- QP 24 Reference Materials
- QP 25 Proficiency Testing
- QP 26 Reviews
- QP 27 Testimony
- QP 28 Reporting of Results
- QP 29 Temperature Monitoring
- QP 30 Consumptive Testing and Observation
- QP 31 Wrongful Action